What are the applications of optical microscopes and electron microscopes?
Optical microscopes are suitable for relatively large substances, and can see objects as small as a dozen microns in size. And the object needs to scatter light relatively well and the depth of field is not large. Can be used to observe cells, bacteria, and large structures of metal tissues.
Electron microscopes can observe objects in the range of several nanometers to tens of microns, and the object under test needs to have good electrical conductivity. Depending on the electron microscope, the objects being observed are different. Scanning electron microscopes mainly observe the surface structure of objects between a few hundred nanometers and tens of microns. The resolution is higher than that of optical microscopes, but lower than that of transmission electron microscopes. It can be used to observe large nanoparticles, finer metal structures, and nanostructures of organisms.
Transmission electron microscopy mainly observes thin film samples ranging from a few nanometers to a few microns, with extremely high resolution. It can be used to observe nanoparticles, metal microstructure and atomic structure.
The difference between chemiluminescence imaging system and gel imaging system
Chemiluminescence is a chemical reaction between two substances A and B to produce substance C. The energy released by the reaction is absorbed by the molecules of substance C and jumps to the excited state C*. The excited C* produces light radiation in the process of returning to the ground state. The difference between gel imaging and chemiluminescence lies in the phenomenon of light radiation accompanying the chemical reaction process, so it is called chemiluminescence. The chemiluminescence imaging system is a plug-and-play all-in-one machine, suitable for chemiluminescence, multi-color fluorescence detection and ordinary gel detection. It uses high-resolution and low-illumination imported refrigeration CCD, and is perfectly combined with a large aperture electric lens to capture Very weak fluorescence and chemiluminescence signals. The deep-cooled CCD of the chemiluminescence imaging system eliminates background noise to the greatest extent, and the ultra-large aperture electric lens collects weak signals. A variety of optional fluorescence light sources and multi-position electric filter wheels can meet various experimental needs such as nucleic acid imaging and ECL imaging.
