Introduction to the Operating Procedures of Optical Microscopes
1. Hold the mirror arm with your right hand and hold the mirror holder with your left hand to keep the mirror body upright. The desktop should be clean and stable, and choose a place near the window or with sufficient light. Single tube items are usually placed on the left side, 3-4 centimeters away from the table edge.
2. Clean and inspect the microscope for any issues, ensure cleanliness, and use a clean soft cloth to wipe the mechanical parts of the mirror body. The lens should be wiped with lens cleaning paper. If there is glue or dirt, a small amount of xylene can be used to clean it.
3. Raise the optical tube to a distance of 1-2 centimeters from the stage, and align the low magnification mirror with the light hole. Adjust the aperture and reflector, use a flat mirror when the light is strong, use a concave mirror when the light is weak, and rotate the reflector with both hands. If a microscope with a light source is used, this step can be omitted, but a knob for adjusting the brightness is required.
4. Install the specimen and place the glass slide on the stage, ensuring that the side with the cover glass is facing upwards. Fix the glass slide with a spring clip and rotate the knob of the platform mover to align the material to be observed with the center of the light hole.
5. When focusing, first rotate the coarse focusing knob to slowly lower the lens barrel, and carefully observe from the side until the objective lens is close to the glass slide specimen. Then, use the left eye to observe from the eyepiece, and use the left hand to rotate the coarse focusing knob to lift the lens barrel until the specimen image is clearly seen, and then use the fine focusing knob to adjust it back clearly. Operation precautions: Do not focus directly under high magnification lenses; When the tube descends, the distance between the tube and the specimen should be observed from the side; To understand the critical value of object distance. If using a binocular microscope, if there is a difference in visual acuity between the observer's eyes, the reliable visual acuity adjustment ring should be used. In addition, the binoculars can be relatively translated to adapt to the distance between the operator's eyes.
6. If using a single tube microscope, naturally open both eyes, observe the specimen with the left eye, record and draw with the right eye, and adjust the focal length with the left hand to make the object clear and move the specimen field of view. Record and draw with your right hand. During microscopic examination, the specimen should be moved in a certain direction until the entire specimen is observed, so as not to miss or repeat the examination. Adjustment of light intensity: In general, the light for stained specimens should be strong, while the light for colorless or unstained specimens should be weak; Low magnification lenses should observe weak light, while high magnification lenses should observe strong light. In addition to adjusting the reflector or light source, adjusting the iris aperture is also very important.
