What is a Confocal microscopy?
Confocal microscopy is an optical imaging technology, which can improve the optical resolution and contrast of microscopic images by using spatial pinholes to block the defocus light. In image formation. Capturing multiple two-dimensional images at different depths in the sample can reconstruct the three-dimensional structure (this process is called optical slicing). This technology is widely used in the scientific and industrial fields, with typical applications being life sciences, semiconductor inspection, and materials science.
The Confocal microscopy is generally a fluorescence microscope and a confocal microscope.
Fluorescent microscope is mainly used in biological field and medical research, which can obtain fluorescent images of the internal microstructure of cells or tissues, observe physiological signals such as Ca2+, PH value, Membrane potential and changes in cell morphology at the subcellular level, and is a new generation of powerful research tools in morphology, molecular biology, neuroscience, pharmacology, genetics and other fields. The Confocal microscopy based on confocal technology is used to measure the surface of various precision devices and materials at the micro and nano level.
The goal of materials science is to study the influence of material surface structure on its surface properties. Therefore, high-resolution analysis of surface morphology is of great significance for determining relevant parameters such as surface roughness, reflective properties, tribological properties, and surface quality. Confocal technology can measure various materials with surface reflection characteristics and obtain effective measurement data.
Confocal microscopy is based on confocal microscopy technology, combined with precision Z-scan module, 3D modeling algorithm, etc., which can perform non-contact scanning on device surface and establish 3D image of the surface to realize 3D measurement of device surface topography. In the field of material production testing, it is possible to measure and analyze the surface morphology characteristics of various products, components, and materials, including surface profile, surface defects, wear, corrosion, flatness, roughness, waviness, pore gap, step height, bending deformation, and processing conditions.
